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You can easily view the expression of 99 genes obtained from single-molecule fluorescence in situ hybridization (smFISH) for wheat apex at 3 stages (early transition (W1.5), late double ridge (W2.5) and floret primordia (W3.5) stages; each stage has two images). Please check the reference below for details of the smFISH experiment. Below is just a quick guide:

1. On the left panel (Cluster Map), you can view all or a few clusters of the smFISH.
2. One the right panel (Expression Heatmap), you can check single gene or a pair of gene expression in each cell of the 6 smFISH images.
3. "Gene 1" has heatmap for the cell fill color; "Gene 2" has heatmap for the cell border color.
4. Select "Gene 1" or "Gene 2" will clear the cluster colors; and "Gene 1" compete the color fill colors with clusters.
5. Gene 2 and the clusters can be shown at the same time, but make sure to select "Gene 2" first, then select the clusters.
6. You can use the mouse wheel to zoom in or out; drag it to move.
7. You can download a SVG (Scalable Vector Graphics) format image, view it with a web browser, and edit it with Inkscape (free) or SVG-edit (free) or Adobe Illustrator (commercial).
8. You can also download a high-quality PNG image. Increase the quality number to get an image with higher quality.
9. You can download Kronos and Chinese spring gene IDs of the 99 genes.

Reference

Xu, X., Lin, H., Zhang, J. et al. Spatial and single-cell expression analyses reveal complex expression domains in early wheat spike development. Genome Biol 26, 352 (2025). https://doi.org/10.1186/s13059-025-03811-3