Tools

Barley Imputed spike smFISH

Imputed JD99 Wheat spike smFISH

JD99 Wheat spike smFISH

Plant pegRNA designer

Multiple sequence alignments

Demultiplex long reads FASTQ files

Map long reads with minimap2

Concatenate files

Gene ID conversion between Kronos and CS

Find wheat potential homeologs

Extract cDNA sequences

Short read de novo assembly with Velevet

CRISPR editing check with BWA and SAMTOOLS v2

CRISPR NGS editing check with HISAT2

Make bam files with strobeAlign

Shade sequence alignments with nimBoxshade

Shade sequence alignments with Boxshade

Design KASP primers with Primer3 v2.5.0

Convert a vcf file to SNP table

Call variants with exactSNP

Index bams with SAMTOOLS

Format FASTA to fixed length

Make bams and indel calls with Subread

Filter fastq files with FASTP

Filter multiple fastq files with FASTP

Make bam files with BWA and SAMTOOLS

CRISPR Editing Analysis v2

Demultiplex a Fastq File

CRISPR Editing Analysis

Call SNPs from multiple sequence alignment

Reverse complement of DNA sequences